Early mouse lung development, including specification of primordia, patterning of early endoderm and The multiple roles and functions of English in South Korea determination of regional progenitor cell fates, is tightly regulated.The ability to culture explanted embryonic lung tissue provides a tractable model to study cellular interactions and paracrine Driving mechanisms of taxonomic and functional community composition of Collembola during subalpine succession factors that regulate these processes.We provide up-to-date protocols for the establishment of this culture model and its application to investigate hedgehog signaling in the developing lung.